Offal marker proteins - extraction and semi-quantification

Description

Standard operating procedure for the extraction of offal marker proteins in raw and processed meat products, and their subsequent detection and semi-quantification

Scope

To probe Western blots of extracts of meat products with anti-heart specific protein 2 (H-SP2), anti-liver specific protein 3 (L-SP3), anti-lung specific protein 2 (Lu-SP2) and anti-kidney marker protein 1 (K-MP1) antibodies in order to detect and semi-quantify added bovine/ovine/porcine heart, liver, lung and kidney, respectively. K-MP1 is a protein expressed at high levels in kidney, small intestine and placenta and at lower levels in liver, pancreas, prostate, spleen, stomach and large intestine, however, it is not expressed in skeletal muscle or heart. The SOP can be used to analyse raw and cooked meat products.

Origin

DEFRA SOP project FA0122

Version

SOP FA0122(3) Version 1.2, January 2012

Author(s)

Prepared by Lyndsey Durose, Nottingham Trent University Date January 2016

Approval status

Reviewed by Authenticity Methods Working Group Date January 2016

Copyright

Contains public sector information licensed under the Open Government Licence v3.0.

File Name: FA0122_14314_SOPFA0122_v1-2.pdf
File Size: 281.09 KB
File Type: application/pdf
Created Date: 11-08-2018
Last Updated Date: 11-08-2018