SOP Basmati Quant Indel Bioanalyser

Description

Standard operating procedure for the quantitative analysis of adulteration of basmati rice with the varieties Sherbati or Pak 386 using the Agilent 2100 Bionalyser and a PCR marker based on an INDEL sequence

Scope

The procedure should be used to test samples for which the presence of adulteration has been detected, and where a second estimate of levels of adulteration with Pak 386 or Sherbati is required. Note that if the RM201 quantification detects >7% adulteration but this test with INDEL B8 does not detect significant adulteration the adulterant variety could be Superfine. Conversely, if this test detects > 7% adulteration but the RM201 test does not, the adulterant variety could be Supra.
The method is designed to quantify the level of non-permitted varieties (Sherbati or PAK 386) in rice grain samples using a PCR marker based on an INDEL sequence.
This method is suitable for the quantitative analysis of known adulterant varieties in a sample of uncooked or par-boiled samples rice grains.
The test can not detect the following non-permitted varieties: Yamini, Pusa 1121, Mugad Sugandha, Superfine, Basmati 2000 and Shaheen Basmati.

Author(s)

Prepared by Katherine Steele, Food DNA Services Date 18 February 2008
Amended by Alison Colyer Date 16 May 2008

Approval status

Information only

Copyright

Contains public sector information licensed under the Open Government Licence v3.0.

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Created Date: 01-31-2016
Last Updated Date: 02-01-2016