Horse relative quantitation - Version 2.1


The method described uses real-time PCR to quantitate the amount of horse DNA relative to the amount of total mammalian DNA extracted from a raw meat sample. The method has been applied to and is validated for DNA extracted from samples that consist of raw horse meat in a raw beef (meat) background. Results can be expressed relative to the amount of raw horse meat in a raw beef (meat) background on a gravimetric (w/w) basis.


DEFRA Standard Operating Procedure - Horse Relative Quantitation

Publication version

Draft Version 2.1, November 2014

Approval status

Project report approved for publication.


Contains public sector information licensed under the Open Government Licence v3.0.

Additional information

A later, unapproved, version of this SOP is available Here

File Name: Horse_RQ _SOP.pdf
File Size: 348.31 KB
File Type: application/pdf
Created Date: 06-17-2015
Last Updated Date: 02-01-2016